Tosoh’s advanced HPLC systems are fully automated systems that rapidly and precisely separate hemoglobins found naturally in human blood. Suitable for high throughput HbA1c testing in the laboratory or clinic environment in addition to rapid Hemoglobinopathy screening, our HPLC solutions provide a reliable, accurate and precise service at all times.
The key applications of their analysis modes are as follows:
Follow-up of diabetic patients
HPLC is considered the “Gold Standard” technology in the follow-up of the plasma glucose concentration of diabetic patients over time, via the measurement of HbA1c (= glycated hemoglobin fraction). This technique was used in the ground breaking DCCT (Diabetes Control and Complications Trial) study.
To follow-up the plasma glucose concentrations over time the “HbA1c analysis mode” should be used. In this mode pre-determined windows are set in the software to detect the presence of Hemoglobins A1a, A1b, F, l-A1c, s-A1c and A0. Calibration against DCCT (NGSP) or IFCC allows the quantitation of the stabile A1c fraction, with other windows allowing presumptive identification of various variant haemoglobins. Read more about diabetes management.
Screening for β-thalassemia and hemoglobinopathies
In the aid of diagnosing β-thalassemia and hemoglobinopathies the “β-thalassemia analysis mode” should be used. In this mode pre-determined windows are set in the software to detect the presence of Hemoglobins F, A0, A2, D+, S+ and C+. Calibration allows the quantitation of HbF and HbA2, with all other windows allowing the presumptive identification of various variant haemoglobins. Read more about hemoglobopathies.
For Gold Standard Ion-exchange HbA1c testing by Tosoh Bioscience, go to Tosoh G8 HPLC Analyzer.