HPLC Testing » hba1c

HPLC Testing: G8 HPLC Analyzer Provides High Precision with Low CVs

admin — Wed, 29 Apr 2009 01:49:55 +0000

Tosoh Bioscience HPLC

(April 28, 2009) … HPLC Testing: Tosoh G8 HPLC Analyzer provides high precision with low CVs. Utilizing the gold standard ion-exchange method of HbA1c measurement, Tosoh’s G8 HPLC Analyzer processes results quickly with less than 2% CVs.

G8 HPLC Analyzer
- HbA1c analysis time – 1.6 minutes
- Direct determination of stable HbA1c
- Less than 2% CVs
- Simple touch-screen operation
- Compact footprint (21”w x 20”d x 19”h)
- NGSP certified
- Low maintenance
- Flexible tube sizes
- 90 Sample Loader (Optional 290 Sample Loader available)

HPLC Testing: G7 HPLC Analyzer Tests Both HbA1c and Beta Thalassemia

admin — Wed, 29 Apr 2009 01:37:10 +0000

(April 28, 2009) … HPLC Testing Report: Tosoh’s G7 HPLC Analyzer tests both beta thalassemia and hemoglobin A1c in one compact benchtop analyzer. Features include: stable HbA1c results in 2.2 minutes, β-thalassemia results in less than 8 minutes, quantification of HbF and HbA2, multi-functional STAT sample position, presumptive ID of hemoglobinopathies, inventory management system, user-friendly software, compact size, NGSP certified.

For more information, go to G7 HPLC Analyzer

HPLC Testing: Tosoh G8 HPLC Analyzer Provides Advancements in HPLC Testing – VIDEO

admin — Tue, 03 Mar 2009 12:19:09 +0000

HPLC Testing by Tosoh Bioscience

(March 3, 2009) - High performance liquid chromatography (HPLC) is the gold standard methodology for the HbA1c test. By manipulating the flow rate of the liquid (i.e. mobile phase) and the temperature of the HPLC column (i.e. stationary phase), it is possible to change the chromatographic display.

The Tosoh G8 HPLC Analyzer provides direct determination of stable HbA1c. The system is used for in vitro diagnostic measurement of HbA1c in blood specimens. The G8 uses a non-porous column and microcomputer technology to quickly and accurately measure the stable portion of HbA1c as a percentage of the total amount of hemoglobin present in the sample. The G8 provides accurate and precise separation of HbA1c from other hemoglobin fractions. Off-line pretreatment is not required, and there is no interference from the labile portion of HbA1c.

The analyzer dilutes the whole blood specimen with Hemolysis & Wash Solution and then injects a small volume of the treated specimen onto the TSKgel Glyco HSi Variant Column. Separation is achieved by utilizing differences in ionic interactions between the cation exchange group on the column resin surface and the hemoglobin components in a step gradient elution. The hemoglobin fractions (designated as A1a, A1b, F, LA1c , SA1c, A0, and H-V0, H-V1, H-V2) are subsequently removed from the column material by performing a step-wise elution using Elution Buffers HSi Variant 1, 2, and 3 that have specific salt and pH concentrations.

The separated hemoglobin components pass through the LED photometer flow cell where changes in absorbance are measured at 415nm. The G8 software integrates and reduces the raw data, and then calculates the relative percentages of each hemoglobin fraction. The print-out consists of the numerical results and the chromatogram. This represents the changes in absorbance versus retention time for each peak fraction. An analysis requires only 1.6 minutes.

HPLC Solutions: Tosoh Bioscience